Abstracts
Use of Protien Profiling Technologies In A Preliminary Assessment Of The Chemotherapeutic Effects Of Parthhenolide.
Advances in genomics have allowed researchers to study cancer through the identification and isolation of genes that control cell growth. However, in order to obtain a more comprehensive understanding of what goes on inside a cancer cell, one must examine a different class of biological molecules that carry out cellular processes: protien. Differential Fluorecence Gel Electrophoresis (DIGE) is one of the protien profiling approaches routinely used in cell culture to identify and compare protiens. This research study used the DIGE process to study a cell line with a temperature-sensitive mutation in the p53 gene product. Cells that were grown in different temperarure conditions were found to exhibit differences in the typse and levels of protien expression. As an attempt to address the molecular basis of the p53 differential response in this cell line, the cytotoxic effect of parthenolide was monitored at the protien level using the DIGE approach.
Studies of Selective Cytotoxicity to Experimental Treatments with Rapamycin and Parthenolide in a Transformed Murine Cell Line GC-2SPD Containing a Temperature-Sensative Mutation in the p53 Gene.
The primary focus of this research was to evaluate and interpret the cytotoxic effects of Parthenolide and rapamycin on a solid and monolayer tumor malignancy in a transformed murine (mouse) cell line called GC-2Spd containing a genetically engineered temperature sensitive mutation in the p53 gene product.